Energy filtered TEM micrograph of yttria (in green) - zirconia (in red) multilayers, Chanchal Ghosh,  IGCAR, Kalpakkam, India  Diatoms World, Mostafa Moonir Shawrav, Institute of Solid State Electronics, Austria 1- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland
Procapsid and nucleocapsid of dsRNA bacteriophage phi6, D. Nemecek, CryoEM Research Group CEITEC, Czech Republic Chroococcus giganteus - Jan Stastny, Charles University, Věda je krásnáPhragmites communis - Jan Martinek, Charles University, Věda je krásná
Orchid root with Mycorrhiza, S. R. Senthilkumar, St. Joseph´s College, India SEM image of ink-bottle silica nanopores, A. Sterczynska,NanoBioMediacl Centre (CNBM), Poznan, Poland Cross section of an Abutilon leaf, Adriana Dominguez and Eduardo Favret, CNEA - INTA, Argentina
Salt, TESCAN2- CVD grown diamond film, Magdalena Parlinska, University of Rzeszow, Poland Offretite Scagno, TESCAN
Rust fungus spore,Adriana Dominguez and Eduardo Favret, CNEA - INTA, Argentina.jpg Scabiosa columbaria - Viktor Sykora, Charles University, Věda je krásnáPolymer fibers, TESCAN
PbI2 Crystallization, TESCANPaulinella chromatophora - Yvonne Nemcova, Charles University, Věda je krásnáPowder metallurgy substrate, TESCAN
2- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland 3- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland 1- CVD grown diamond film, Magdalena Parlinska, University of Rzeszow, Poland

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Cryo-Ultramicrotomy in Biology

Organizer: Helmut Gnaegi, Diatome

Contact: helmut.gnaegi@diatome.ch

Abstract:Cryo-ultramicrotomy gains increasing interest in the life science community. The sample preparation by plunge freezing or high pressure freezing, cryoultramicrotomy, cryo-transfer and cryo-TEM preserve living matter close to the native state. The cutting process is accompanied by a number of artefacts and problems. A crucial part in cryo-sectioning is the section pick-up. The transfer of the sections onto the grid is difficult and new micromanipulators for the handling of the section ribbons, which make it easier and less depending on the operator's skill, will be demonstrated. Possible scenarios for reducing these artefacts will be discussed.

Date: half day workshop, September 7, 2014 from 1 PM to 4:30 PM

Venue: Prague Congress Centre, Prague 4

Minimal number of participants: 20

Registration fee: 40 EUR (included one coffee break and workshop materials)

 

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