Paulinella chromatophora - Yvonne Nemcova, Charles University, Věda je krásnáGold on Germanium, Benedykt R. Jany, Marian SmoluchowskiInstitute of Physics - Jagiellonian University, Poland SEM image of ink-bottle silica nanopores, A. Sterczynska,NanoBioMediacl Centre (CNBM), Poznan, Poland
Rotaviruses, Electronmicroscopy, Elisabeth M. Schraner, Institutes of Vet. Anatomy and Virology, Switzerland 2- CVD grown diamond film, Magdalena Parlinska, University of Rzeszow, Poland 1- Diatom, Magdalena Parlinska, University of Rzeszow, Poland
Plasma coating crossection, TESCANPollen grain of Hyoseris radiata, TESCAN2- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland
Powder metallurgy substrate, TESCANOrchid root with Mycorrhiza, S. R. Senthilkumar, St. Joseph´s College, India Orchid root stained with Acridine orange, S. R. Senthilkumar, St. Joseph´s College, India
1- CVD grown diamond film, Magdalena Parlinska, University of Rzeszow, Poland Diatoms World, Mostafa Moonir Shawrav, Institute of Solid State Electronics, Austria 2- Diatom, Magdalena Parlinska, University of Rzeszow, Poland
Eudorina - Pavel Skaloud, Charles University, Věda je krásnáOrchid root showing with idioblastic cells, S. R. Senthilkumar, St. Joseph´s College, India Offretite Scagno, TESCAN
1- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland Coral, TESCANButterfly Wings, Benedykt R. Jany, Marian SmoluchowskiInstitute of Physics - Jagiellonian University, Poland

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Cryo-Ultramicrotomy in Biology

Organizer: Helmut Gnaegi, Diatome

Contact: helmut.gnaegi@diatome.ch

Abstract:Cryo-ultramicrotomy gains increasing interest in the life science community. The sample preparation by plunge freezing or high pressure freezing, cryoultramicrotomy, cryo-transfer and cryo-TEM preserve living matter close to the native state. The cutting process is accompanied by a number of artefacts and problems. A crucial part in cryo-sectioning is the section pick-up. The transfer of the sections onto the grid is difficult and new micromanipulators for the handling of the section ribbons, which make it easier and less depending on the operator's skill, will be demonstrated. Possible scenarios for reducing these artefacts will be discussed.

Date: half day workshop, September 7, 2014 from 1 PM to 4:30 PM

Venue: Prague Congress Centre, Prague 4

Minimal number of participants: 20

Registration fee: 40 EUR (included one coffee break and workshop materials)

 

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