Paulinella chromatophora - Yvonne Nemcova, Charles University, Věda je krásnáEudorina - Pavel Skaloud, Charles University, Věda je krásnáSalt, TESCAN
Rhaphoneis - Pavel Skaloud, Charles University, Věda je krásnáPlasma coating crossection, TESCAN3- Diatom, Magdalena Parlinska, University of Rzeszow, Poland
Pollen grain of Hyoseris radiata, TESCANRust fungus spore,Adriana Dominguez and Eduardo Favret, CNEA - INTA, Argentina.jpg Leaf Fract, TESCAN
4- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland 2- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland Procapsid and nucleocapsid of dsRNA bacteriophage phi6, D. Nemecek, CryoEM Research Group CEITEC, Czech Republic
Rotaviruses, Electronmicroscopy, Elisabeth M. Schraner, Institutes of Vet. Anatomy and Virology, Switzerland Orchid root stained with Acridine orange, S. R. Senthilkumar, St. Joseph´s College, India Scabiosa columbaria - Viktor Sykora, Charles University, Věda je krásná
Powder metallurgy substrate, TESCANChroococcus giganteus - Jan Stastny, Charles University, Věda je krásná1- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland
Phragmites communis - Jan Martinek, Charles University, Věda je krásnáPolymer fibers, TESCAN3- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland

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Cryo-Ultramicrotomy in Biology

Organizer: Helmut Gnaegi, Diatome

Contact: helmut.gnaegi@diatome.ch

Abstract:Cryo-ultramicrotomy gains increasing interest in the life science community. The sample preparation by plunge freezing or high pressure freezing, cryoultramicrotomy, cryo-transfer and cryo-TEM preserve living matter close to the native state. The cutting process is accompanied by a number of artefacts and problems. A crucial part in cryo-sectioning is the section pick-up. The transfer of the sections onto the grid is difficult and new micromanipulators for the handling of the section ribbons, which make it easier and less depending on the operator's skill, will be demonstrated. Possible scenarios for reducing these artefacts will be discussed.

Date: half day workshop, September 7, 2014 from 1 PM to 4:30 PM

Venue: Prague Congress Centre, Prague 4

Minimal number of participants: 20

Registration fee: 40 EUR (included one coffee break and workshop materials)

 

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