Phragmites communis - Jan Martinek, Charles University, Věda je krásnáChroococcus giganteus - Jan Stastny, Charles University, Věda je krásnáRotaviruses, Electronmicroscopy, Elisabeth M. Schraner, Institutes of Vet. Anatomy and Virology, Switzerland
Collagen fibers in cartilage, E. I. Romijn, NTNU, Trondheim Pollen grain of Hyoseris radiata, TESCAN1- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland
2- Diatom, Magdalena Parlinska, University of Rzeszow, Poland SEM image of ink-bottle silica nanopores, A. Sterczynska,NanoBioMediacl Centre (CNBM), Poznan, Poland PbI2 Crystallization, TESCAN
1- Diatom, Magdalena Parlinska, University of Rzeszow, Poland Eudorina - Pavel Skaloud, Charles University, Věda je krásná3- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland
Gold on Germanium, Benedykt R. Jany, Marian SmoluchowskiInstitute of Physics - Jagiellonian University, Poland Polymer fibers, TESCANCross section of an Abutilon leaf, Adriana Dominguez and Eduardo Favret, CNEA - INTA, Argentina
2- CVD grown diamond film, Magdalena Parlinska, University of Rzeszow, Poland Uniform core shell Fe nanoparticles, S. Bandyopadhyay, NTNU, Trondheim 1- CVD grown diamond film, Magdalena Parlinska, University of Rzeszow, Poland
Plasma coating crossection, TESCANEnergy filtered TEM micrograph of yttria (in green) - zirconia (in red) multilayers, Chanchal Ghosh,  IGCAR, Kalpakkam, India  Orchid root stained with Acridine orange, S. R. Senthilkumar, St. Joseph´s College, India

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Cryo-Ultramicrotomy in Biology

Organizer: Helmut Gnaegi, Diatome

Contact: helmut.gnaegi@diatome.ch

Abstract:Cryo-ultramicrotomy gains increasing interest in the life science community. The sample preparation by plunge freezing or high pressure freezing, cryoultramicrotomy, cryo-transfer and cryo-TEM preserve living matter close to the native state. The cutting process is accompanied by a number of artefacts and problems. A crucial part in cryo-sectioning is the section pick-up. The transfer of the sections onto the grid is difficult and new micromanipulators for the handling of the section ribbons, which make it easier and less depending on the operator's skill, will be demonstrated. Possible scenarios for reducing these artefacts will be discussed.

Date: half day workshop, September 7, 2014 from 1 PM to 4:30 PM

Venue: Prague Congress Centre, Prague 4

Minimal number of participants: 20

Registration fee: 40 EUR (included one coffee break and workshop materials)

 

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