Rhaphoneis - Pavel Skaloud, Charles University, Věda je krásnáSalt, TESCAN2- CVD grown diamond film, Magdalena Parlinska, University of Rzeszow, Poland
Leaf Fract, TESCANUniform core shell Fe nanoparticles, S. Bandyopadhyay, NTNU, Trondheim Pollen of Lavatera arborea, TESCAN
Scabiosa columbaria - Viktor Sykora, Charles University, Věda je krásnáRust fungus spore,Adriana Dominguez and Eduardo Favret, CNEA - INTA, Argentina.jpg Powder metallurgy substrate, TESCAN
4- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland Polymer fibers, TESCANSEM image of ink-bottle silica nanopores, A. Sterczynska,NanoBioMediacl Centre (CNBM), Poznan, Poland
Orchid root stained with Acridine orange, S. R. Senthilkumar, St. Joseph´s College, India Cross section of an Abutilon leaf, Adriana Dominguez and Eduardo Favret, CNEA - INTA, Argentina Scenedesmus quadricauda - Viktor Sykora, Charles University , Věda je krásná
3- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland Pollen grain of Hyoseris radiata, TESCANChroococcus giganteus - Jan Stastny, Charles University, Věda je krásná
Plasma coating crossection, TESCANDiatoms World, Mostafa Moonir Shawrav, Institute of Solid State Electronics, Austria Collagen fibers in cartilage, E. I. Romijn, NTNU, Trondheim

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Cryo-Ultramicrotomy in Biology

Organizer: Helmut Gnaegi, Diatome

Contact: helmut.gnaegi@diatome.ch

Abstract:Cryo-ultramicrotomy gains increasing interest in the life science community. The sample preparation by plunge freezing or high pressure freezing, cryoultramicrotomy, cryo-transfer and cryo-TEM preserve living matter close to the native state. The cutting process is accompanied by a number of artefacts and problems. A crucial part in cryo-sectioning is the section pick-up. The transfer of the sections onto the grid is difficult and new micromanipulators for the handling of the section ribbons, which make it easier and less depending on the operator's skill, will be demonstrated. Possible scenarios for reducing these artefacts will be discussed.

Date: half day workshop, September 7, 2014 from 1 PM to 4:30 PM

Venue: Prague Congress Centre, Prague 4

Minimal number of participants: 20

Registration fee: 40 EUR (included one coffee break and workshop materials)

 

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