Orchid root with Mycorrhiza, S. R. Senthilkumar, St. Joseph´s College, India Cross section of an Abutilon leaf, Adriana Dominguez and Eduardo Favret, CNEA - INTA, Argentina Eudorina - Pavel Skaloud, Charles University, Věda je krásná
Phragmites communis - Jan Martinek, Charles University, Věda je krásnáPbI2 Crystallization, TESCANSEM image of ink-bottle silica nanopores, A. Sterczynska,NanoBioMediacl Centre (CNBM), Poznan, Poland
Energy filtered TEM micrograph of yttria (in green) - zirconia (in red) multilayers, Chanchal Ghosh,  IGCAR, Kalpakkam, India  Orchid root stained with Acridine orange, S. R. Senthilkumar, St. Joseph´s College, India Powder metallurgy substrate, TESCAN
Scenedesmus quadricauda - Viktor Sykora, Charles University , Věda je krásnáRust fungus spore,Adriana Dominguez and Eduardo Favret, CNEA - INTA, Argentina.jpg Butterfly Wings, Benedykt R. Jany, Marian SmoluchowskiInstitute of Physics - Jagiellonian University, Poland
Collagen fibers in cartilage, E. I. Romijn, NTNU, Trondheim Gold on Germanium, Benedykt R. Jany, Marian SmoluchowskiInstitute of Physics - Jagiellonian University, Poland 3- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland
Plasma coating crossection, TESCANOrchid root showing with idioblastic cells, S. R. Senthilkumar, St. Joseph´s College, India Coral, TESCAN
Diatoms World, Mostafa Moonir Shawrav, Institute of Solid State Electronics, Austria 2- CVD grown diamond film, Magdalena Parlinska, University of Rzeszow, Poland Scabiosa columbaria - Viktor Sykora, Charles University, Věda je krásná

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Cryo-Ultramicrotomy in Biology

Organizer: Helmut Gnaegi, Diatome

Contact: helmut.gnaegi@diatome.ch

Abstract:Cryo-ultramicrotomy gains increasing interest in the life science community. The sample preparation by plunge freezing or high pressure freezing, cryoultramicrotomy, cryo-transfer and cryo-TEM preserve living matter close to the native state. The cutting process is accompanied by a number of artefacts and problems. A crucial part in cryo-sectioning is the section pick-up. The transfer of the sections onto the grid is difficult and new micromanipulators for the handling of the section ribbons, which make it easier and less depending on the operator's skill, will be demonstrated. Possible scenarios for reducing these artefacts will be discussed.

Date: half day workshop, September 7, 2014 from 1 PM to 4:30 PM

Venue: Prague Congress Centre, Prague 4

Minimal number of participants: 20

Registration fee: 40 EUR (included one coffee break and workshop materials)

 

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