Scabiosa columbaria - Viktor Sykora, Charles University, Věda je krásnáGold on Germanium, Benedykt R. Jany, Marian SmoluchowskiInstitute of Physics - Jagiellonian University, Poland 4- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland
Plasma coating crossection, TESCANCoral, TESCAN2- Diatom, Magdalena Parlinska, University of Rzeszow, Poland
2- CVD grown diamond film, Magdalena Parlinska, University of Rzeszow, Poland SEM image of ink-bottle silica nanopores, A. Sterczynska,NanoBioMediacl Centre (CNBM), Poznan, Poland Rotaviruses, Electronmicroscopy, Elisabeth M. Schraner, Institutes of Vet. Anatomy and Virology, Switzerland
PbI2 Crystallization, TESCANRhaphoneis - Pavel Skaloud, Charles University, Věda je krásnáPaulinella chromatophora - Yvonne Nemcova, Charles University, Věda je krásná
Butterfly Wings, Benedykt R. Jany, Marian SmoluchowskiInstitute of Physics - Jagiellonian University, Poland Cross section of an Abutilon leaf, Adriana Dominguez and Eduardo Favret, CNEA - INTA, Argentina Collagen fibers in cartilage, E. I. Romijn, NTNU, Trondheim
Chroococcus giganteus - Jan Stastny, Charles University, Věda je krásnáPhragmites communis - Jan Martinek, Charles University, Věda je krásná3- Diatom, Magdalena Parlinska, University of Rzeszow, Poland
Polymer fibers, TESCANProcapsid and nucleocapsid of dsRNA bacteriophage phi6, D. Nemecek, CryoEM Research Group CEITEC, Czech Republic Energy filtered TEM micrograph of yttria (in green) - zirconia (in red) multilayers, Chanchal Ghosh,  IGCAR, Kalpakkam, India

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Cryo-Ultramicrotomy in Biology

Organizer: Helmut Gnaegi, Diatome

Contact: helmut.gnaegi@diatome.ch

Abstract:Cryo-ultramicrotomy gains increasing interest in the life science community. The sample preparation by plunge freezing or high pressure freezing, cryoultramicrotomy, cryo-transfer and cryo-TEM preserve living matter close to the native state. The cutting process is accompanied by a number of artefacts and problems. A crucial part in cryo-sectioning is the section pick-up. The transfer of the sections onto the grid is difficult and new micromanipulators for the handling of the section ribbons, which make it easier and less depending on the operator's skill, will be demonstrated. Possible scenarios for reducing these artefacts will be discussed.

Date: half day workshop, September 7, 2014 from 1 PM to 4:30 PM

Venue: Prague Congress Centre, Prague 4

Minimal number of participants: 20

Registration fee: 40 EUR (included one coffee break and workshop materials)

 

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