Plasma coating crossection, TESCANSEM image of ink-bottle silica nanopores, A. Sterczynska,NanoBioMediacl Centre (CNBM), Poznan, Poland Powder metallurgy substrate, TESCAN
Butterfly Wings, Benedykt R. Jany, Marian SmoluchowskiInstitute of Physics - Jagiellonian University, Poland 4- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland Paulinella chromatophora - Yvonne Nemcova, Charles University, Věda je krásná
Orchid root showing with idioblastic cells, S. R. Senthilkumar, St. Joseph´s College, India 1- CVD grown diamond film, Magdalena Parlinska, University of Rzeszow, Poland Offretite Scagno, TESCAN
2- CVD grown diamond film, Magdalena Parlinska, University of Rzeszow, Poland Leaf Fract, TESCAN1- Diatom, Magdalena Parlinska, University of Rzeszow, Poland
Coral, TESCANPollen grain of Hyoseris radiata, TESCANRotaviruses, Electronmicroscopy, Elisabeth M. Schraner, Institutes of Vet. Anatomy and Virology, Switzerland
Rhaphoneis - Pavel Skaloud, Charles University, Věda je krásná2- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland Chroococcus giganteus - Jan Stastny, Charles University, Věda je krásná
3- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland Orchid root with Mycorrhiza, S. R. Senthilkumar, St. Joseph´s College, India Diatoms World, Mostafa Moonir Shawrav, Institute of Solid State Electronics, Austria

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Cryo-Ultramicrotomy in Biology

Organizer: Helmut Gnaegi, Diatome

Contact: helmut.gnaegi@diatome.ch

Abstract:Cryo-ultramicrotomy gains increasing interest in the life science community. The sample preparation by plunge freezing or high pressure freezing, cryoultramicrotomy, cryo-transfer and cryo-TEM preserve living matter close to the native state. The cutting process is accompanied by a number of artefacts and problems. A crucial part in cryo-sectioning is the section pick-up. The transfer of the sections onto the grid is difficult and new micromanipulators for the handling of the section ribbons, which make it easier and less depending on the operator's skill, will be demonstrated. Possible scenarios for reducing these artefacts will be discussed.

Date: half day workshop, September 7, 2014 from 1 PM to 4:30 PM

Venue: Prague Congress Centre, Prague 4

Minimal number of participants: 20

Registration fee: 40 EUR (included one coffee break and workshop materials)

 

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