Energy filtered TEM micrograph of yttria (in green) - zirconia (in red) multilayers, Chanchal Ghosh,  IGCAR, Kalpakkam, India  Collagen fibers in cartilage, E. I. Romijn, NTNU, Trondheim Pollen grain of Hyoseris radiata, TESCAN
Chroococcus giganteus - Jan Stastny, Charles University, Věda je krásnáOrchid root showing with idioblastic cells, S. R. Senthilkumar, St. Joseph´s College, India Plasma coating crossection, TESCAN
Leaf Fract, TESCANRotaviruses, Electronmicroscopy, Elisabeth M. Schraner, Institutes of Vet. Anatomy and Virology, Switzerland Powder metallurgy substrate, TESCAN
1- Diatom, Magdalena Parlinska, University of Rzeszow, Poland Scenedesmus quadricauda - Viktor Sykora, Charles University , Věda je krásná2- Diatom, Magdalena Parlinska, University of Rzeszow, Poland
Paulinella chromatophora - Yvonne Nemcova, Charles University, Věda je krásnáDiatoms World, Mostafa Moonir Shawrav, Institute of Solid State Electronics, Austria Rhaphoneis - Pavel Skaloud, Charles University, Věda je krásná
2- CVD grown diamond film, Magdalena Parlinska, University of Rzeszow, Poland 2- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland Pollen of Lavatera arborea, TESCAN
Cross section of an Abutilon leaf, Adriana Dominguez and Eduardo Favret, CNEA - INTA, Argentina 4- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland Eudorina - Pavel Skaloud, Charles University, Věda je krásná

home   registration   submission   accomodation   social   exhibitors   tours

  


Thursday, 11 September
Time: 12:30 - 13:45

GE Healthcare (45 min)
Room: Meeting Hall 4 (2nd floor)

12.30 - 12.45 Welcome Lunch Provided

12.45 - 13.30 Practical Approaches and Considerations for Super-resolution Imaging. Daniel J White Ph.D. & Przemyslaw Fleszar, Field Applications Scientist GE Healthcare Life Sciences.

3D-SIM and Localization microscopy are powerful techniques for cell biologists. When viewed with a microscope, the signal from any sub-diffraction-limit object (e.g., a single fluorescent molecule) will be blurred because of diffraction, thus causing the object to appear larger than its actual size. Recently a variety of techniques have been developed to allow researchers to be able to get a closer look at the actual objects size and location. Collectively these techniques are referred to as Optical Super-resolution.

3D-SIM is a technique that utilizes a structured light pattern and image reconstruction to gain a two-fold resolution improvement in XY and Z. Localization microscopy represents a combination of imaging and mathematical modelling, which can be used to precisely determine the position of fluorophores in a biological sample. GE Healthcare's DeltaVision Microscopy Systems offer options for both these imaging modalities. However instrumentation is only part of the solution. Electron microscopists have known for decades that the more resolution you seek the more the characteristics of the sample and preparation methods become critical. Our presentation will give a brief overview of 3D-SIM and Localization Microscopy, discussing the practical considerations necessary to obtain the best results from optical super-resolution techniques and when to use them.

13.30 - 13.45 Questions

 

 





 

News

PLEASE GO TO THE
Gate

Individual

Proceedings

PRE AND POST congress activities

JOIN US FOR CONGRESS DINNER

CONGRESS POSTER


Vote for the best
micrograph in
"ART IN MICROSCOPY
"

Take a microscopy walk

DETAILED PROGRAM



IMC 2014 comming soon:

Deadlines

5 September 2014
- Vote for the Vox Populi Award

Media Partners







animation
   
 
Intranet