2- CVD grown diamond film, Magdalena Parlinska, University of Rzeszow, Poland Butterfly Wings, Benedykt R. Jany, Marian SmoluchowskiInstitute of Physics - Jagiellonian University, Poland Gold on Germanium, Benedykt R. Jany, Marian SmoluchowskiInstitute of Physics - Jagiellonian University, Poland
Powder metallurgy substrate, TESCAN3- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland Scenedesmus quadricauda - Viktor Sykora, Charles University , Věda je krásná
Leaf Fract, TESCANOffretite Scagno, TESCAN3- Diatom, Magdalena Parlinska, University of Rzeszow, Poland
PbI2 Crystallization, TESCANCoral, TESCANSEM image of ink-bottle silica nanopores, A. Sterczynska,NanoBioMediacl Centre (CNBM), Poznan, Poland
Procapsid and nucleocapsid of dsRNA bacteriophage phi6, D. Nemecek, CryoEM Research Group CEITEC, Czech Republic 4- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland Plasma coating crossection, TESCAN
Salt, TESCANPaulinella chromatophora - Yvonne Nemcova, Charles University, Věda je krásnáPolymer fibers, TESCAN
Rhaphoneis - Pavel Skaloud, Charles University, Věda je krásná2- Diatom, Magdalena Parlinska, University of Rzeszow, Poland Energy filtered TEM micrograph of yttria (in green) - zirconia (in red) multilayers, Chanchal Ghosh,  IGCAR, Kalpakkam, India

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Thursday, 11 September
Time: 12:30 - 13:45

GE Healthcare (45 min)
Room: Meeting Hall 4 (2nd floor)

12.30 - 12.45 Welcome Lunch Provided

12.45 - 13.30 Practical Approaches and Considerations for Super-resolution Imaging. Daniel J White Ph.D. & Przemyslaw Fleszar, Field Applications Scientist GE Healthcare Life Sciences.

3D-SIM and Localization microscopy are powerful techniques for cell biologists. When viewed with a microscope, the signal from any sub-diffraction-limit object (e.g., a single fluorescent molecule) will be blurred because of diffraction, thus causing the object to appear larger than its actual size. Recently a variety of techniques have been developed to allow researchers to be able to get a closer look at the actual objects size and location. Collectively these techniques are referred to as Optical Super-resolution.

3D-SIM is a technique that utilizes a structured light pattern and image reconstruction to gain a two-fold resolution improvement in XY and Z. Localization microscopy represents a combination of imaging and mathematical modelling, which can be used to precisely determine the position of fluorophores in a biological sample. GE Healthcare's DeltaVision Microscopy Systems offer options for both these imaging modalities. However instrumentation is only part of the solution. Electron microscopists have known for decades that the more resolution you seek the more the characteristics of the sample and preparation methods become critical. Our presentation will give a brief overview of 3D-SIM and Localization Microscopy, discussing the practical considerations necessary to obtain the best results from optical super-resolution techniques and when to use them.

13.30 - 13.45 Questions

 

 





 

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