Diatoms World, Mostafa Moonir Shawrav, Institute of Solid State Electronics, Austria 2- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland Leaf Fract, TESCAN
Polymer fibers, TESCAN1- CVD grown diamond film, Magdalena Parlinska, University of Rzeszow, Poland Energy filtered TEM micrograph of yttria (in green) - zirconia (in red) multilayers, Chanchal Ghosh,  IGCAR, Kalpakkam, India
Orchid root showing with idioblastic cells, S. R. Senthilkumar, St. Joseph´s College, India Procapsid and nucleocapsid of dsRNA bacteriophage phi6, D. Nemecek, CryoEM Research Group CEITEC, Czech Republic Cross section of an Abutilon leaf, Adriana Dominguez and Eduardo Favret, CNEA - INTA, Argentina
Uniform core shell Fe nanoparticles, S. Bandyopadhyay, NTNU, Trondheim Orchid root with Mycorrhiza, S. R. Senthilkumar, St. Joseph´s College, India Phragmites communis - Jan Martinek, Charles University, Věda je krásná
Pollen of Lavatera arborea, TESCAN1- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland Rust fungus spore,Adriana Dominguez and Eduardo Favret, CNEA - INTA, Argentina.jpg
Collagen fibers in cartilage, E. I. Romijn, NTNU, Trondheim Offretite Scagno, TESCAN3- Diatom, Magdalena Parlinska, University of Rzeszow, Poland
Gold on Germanium, Benedykt R. Jany, Marian SmoluchowskiInstitute of Physics - Jagiellonian University, Poland Powder metallurgy substrate, TESCANRotaviruses, Electronmicroscopy, Elisabeth M. Schraner, Institutes of Vet. Anatomy and Virology, Switzerland

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Thursday, 11 September
Time: 12:30 - 13:45

GE Healthcare (45 min)
Room: Meeting Hall 4 (2nd floor)

12.30 - 12.45 Welcome Lunch Provided

12.45 - 13.30 Practical Approaches and Considerations for Super-resolution Imaging. Daniel J White Ph.D. & Przemyslaw Fleszar, Field Applications Scientist GE Healthcare Life Sciences.

3D-SIM and Localization microscopy are powerful techniques for cell biologists. When viewed with a microscope, the signal from any sub-diffraction-limit object (e.g., a single fluorescent molecule) will be blurred because of diffraction, thus causing the object to appear larger than its actual size. Recently a variety of techniques have been developed to allow researchers to be able to get a closer look at the actual objects size and location. Collectively these techniques are referred to as Optical Super-resolution.

3D-SIM is a technique that utilizes a structured light pattern and image reconstruction to gain a two-fold resolution improvement in XY and Z. Localization microscopy represents a combination of imaging and mathematical modelling, which can be used to precisely determine the position of fluorophores in a biological sample. GE Healthcare's DeltaVision Microscopy Systems offer options for both these imaging modalities. However instrumentation is only part of the solution. Electron microscopists have known for decades that the more resolution you seek the more the characteristics of the sample and preparation methods become critical. Our presentation will give a brief overview of 3D-SIM and Localization Microscopy, discussing the practical considerations necessary to obtain the best results from optical super-resolution techniques and when to use them.

13.30 - 13.45 Questions

 

 





 

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