Orchid root showing with idioblastic cells, S. R. Senthilkumar, St. Joseph´s College, India 2- Diatom, Magdalena Parlinska, University of Rzeszow, Poland Rhaphoneis - Pavel Skaloud, Charles University, Věda je krásná
Pollen grain of Hyoseris radiata, TESCANPolymer fibers, TESCANRust fungus spore,Adriana Dominguez and Eduardo Favret, CNEA - INTA, Argentina.jpg
SEM image of ink-bottle silica nanopores, A. Sterczynska,NanoBioMediacl Centre (CNBM), Poznan, Poland Uniform core shell Fe nanoparticles, S. Bandyopadhyay, NTNU, Trondheim 3- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland
Diatoms World, Mostafa Moonir Shawrav, Institute of Solid State Electronics, Austria Procapsid and nucleocapsid of dsRNA bacteriophage phi6, D. Nemecek, CryoEM Research Group CEITEC, Czech Republic Orchid root stained with Acridine orange, S. R. Senthilkumar, St. Joseph´s College, India
3- Diatom, Magdalena Parlinska, University of Rzeszow, Poland Scenedesmus quadricauda - Viktor Sykora, Charles University , Věda je krásnáPollen of Lavatera arborea, TESCAN
PbI2 Crystallization, TESCANRotaviruses, Electronmicroscopy, Elisabeth M. Schraner, Institutes of Vet. Anatomy and Virology, Switzerland Offretite Scagno, TESCAN
1- CVD grown diamond film, Magdalena Parlinska, University of Rzeszow, Poland Plasma coating crossection, TESCAN1- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland

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Cryo FIB-SEM in Life Sciences: From Sample Preparation to 3D Volume Rendering



Organizer
: TESCAN & LEICA

Contact: imc2014@tescan.cz

Registration for this workshop is already closed. In case of interest, please contact us directly by e-mail.

Abstract
: Cryo-SEM techniques are becoming a key technology for high resolution visualization of biological samples in their native state. Techniques, such as high pressure freezing enable researchers to create a time snapshot of cellular processes by ultrafast freezing with minimum artifacts. Frozen samples can be either embedded in the resin by freeze substitution or observed directly in the frozen state. Focused ion beam – scanning electron microscopy (FIB-SEM) combines site specific removal of the material using the ion beam with block face SEM imaging of the exposed internal structures. By continuous repeating of the high resolution milling-imaging process, one can generate a high resolution 3D reconstruction of the sample.
This workshop will guide you through the whole process, from the sample preparation steps to the final data rendering. It will be divided into two sessions. In the morning, academic professionals will present lectures on fundamentals and recent research advances in the field of cryo FIB-SEM techniques. The afternoon session will focus on practical demonstrations of the whole workflow. A complete portfolio of instruments will be available for use with guidance, including high pressure freezer, sputter coating, vacuum cryo sample transfer and FIB-SEM instrumentation. Interested individuals may use this event for exploring whether this technology suits their research needs, and discuss their applications with vendor representatives.

Date: one day workshop, September 6, from 9AM to 5PM

Venue: the hotel Holiday Inn (next to the Prague Congress Centre), Prague 4

Maximal number of participants: 30

Registration fee: 95 EUR (included coffee breaks, one lunch and workshop materials)

 

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