Orchid root showing with idioblastic cells, S. R. Senthilkumar, St. Joseph´s College, India Polymer fibers, TESCANGold on Germanium, Benedykt R. Jany, Marian SmoluchowskiInstitute of Physics - Jagiellonian University, Poland
Salt, TESCANEnergy filtered TEM micrograph of yttria (in green) - zirconia (in red) multilayers, Chanchal Ghosh,  IGCAR, Kalpakkam, India  Coral, TESCAN
3- Diatom, Magdalena Parlinska, University of Rzeszow, Poland Eudorina - Pavel Skaloud, Charles University, Věda je krásnáCollagen fibers in cartilage, E. I. Romijn, NTNU, Trondheim
2- CVD grown diamond film, Magdalena Parlinska, University of Rzeszow, Poland Scabiosa columbaria - Viktor Sykora, Charles University, Věda je krásnáOffretite Scagno, TESCAN
Diatoms World, Mostafa Moonir Shawrav, Institute of Solid State Electronics, Austria Procapsid and nucleocapsid of dsRNA bacteriophage phi6, D. Nemecek, CryoEM Research Group CEITEC, Czech Republic PbI2 Crystallization, TESCAN
Uniform core shell Fe nanoparticles, S. Bandyopadhyay, NTNU, Trondheim 1- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland Orchid root stained with Acridine orange, S. R. Senthilkumar, St. Joseph´s College, India
Scenedesmus quadricauda - Viktor Sykora, Charles University , Věda je krásná3- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland Chroococcus giganteus - Jan Stastny, Charles University, Věda je krásná

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Cryo FIB-SEM in Life Sciences: From Sample Preparation to 3D Volume Rendering


Contact: imc2014@tescan.cz

Registration for this workshop is already closed. In case of interest, please contact us directly by e-mail.

: Cryo-SEM techniques are becoming a key technology for high resolution visualization of biological samples in their native state. Techniques, such as high pressure freezing enable researchers to create a time snapshot of cellular processes by ultrafast freezing with minimum artifacts. Frozen samples can be either embedded in the resin by freeze substitution or observed directly in the frozen state. Focused ion beam – scanning electron microscopy (FIB-SEM) combines site specific removal of the material using the ion beam with block face SEM imaging of the exposed internal structures. By continuous repeating of the high resolution milling-imaging process, one can generate a high resolution 3D reconstruction of the sample.
This workshop will guide you through the whole process, from the sample preparation steps to the final data rendering. It will be divided into two sessions. In the morning, academic professionals will present lectures on fundamentals and recent research advances in the field of cryo FIB-SEM techniques. The afternoon session will focus on practical demonstrations of the whole workflow. A complete portfolio of instruments will be available for use with guidance, including high pressure freezer, sputter coating, vacuum cryo sample transfer and FIB-SEM instrumentation. Interested individuals may use this event for exploring whether this technology suits their research needs, and discuss their applications with vendor representatives.

Date: one day workshop, September 6, from 9AM to 5PM

Venue: the hotel Holiday Inn (next to the Prague Congress Centre), Prague 4

Maximal number of participants: 30

Registration fee: 95 EUR (included coffee breaks, one lunch and workshop materials)






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